Effect of a novel antifungal peptide P852 on cell morphology and membrane permeability of Fusarium oxysporum |
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| Authors: | Yuzhu Han Jianjun Zhao Bao Zhang Qian Shen Qingmao Shang Pinglan Li |
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| Institution: | 1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China;2. College of Animal Science, Southwest University, Chongqing, China;3. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China;4. Department of Microbiology, Ohio State University, Columbus, OH, United States |
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| Abstract: | P852, a novel cyclic peptide isolated from Bacillus amyloliquefaciens L-H15, showed potent antifungal activity against several major plant fungal pathogens including Fusarium oxysporum. To elucidate the antifungal mechanism, the impact of P852 on the cell morphology and membrane permeabilization of F. oxysporum was studied. By applying electron microscopy and fluorescent techniques, we showed that P852 treatment caused the morphological change of F. oxysporum cells and disrupted its cell structure, including formation of blebs, broken hyphae, deformation of membrane, intracellular organization disruption, pore formation, and cell lysis. Our findings provide insights into the mode of action of P852, which laying a foundation to develop P852 as a novel antifungal agent to control plant fungal pathogens. |
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| Keywords: | Antifungal peptide Membrane permeabilization Cell morphology |
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