Glufosinate as an efficient inhibitor of callus proliferation in coffee tissue |
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Authors: | Jos Van Boxtel Albertus Eskes Marc Berthouly |
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Affiliation: | (1) Present address: Department of Virus Research, John Innes Centre, Colney Lane, NR4 7UH Norwich, United Kingdom;(2) CIRAD-CP, Programme Café, BP 5035, 34032 Montpellier cédex, France |
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Abstract: | Summary To improve selection of transgenic Coffea spp. tissue after transformation treatments, the effects of the selective agents chlorsulfuron, glufosinate, glyphosate, hygromycin, and kanamycin were studied on callus development from leaf explants (from greenhouse-grown plants and somaplants) and in embryogenic suspension cultures. Studied genotypes were from C. arabica, C. canephora, and the interspecific hybrids Arabusta and Congusta. A culture system based on “direct” somatic embryogenesis from C. canephora leaf explants proved to be more sensitive to selective agents than high frequency somatic embryogenesis from C. arabica or Arabusta leaf explants. With respect to the selective effect, chlorsulfuron and hygromycin provoked strong inhibition and severe necrosis, whereas glyphosate and kanamycin showed variable inhibition. Glufosinate appeared to efficiently inhibit growth of both leaf callus and callus suspensions of all genotypes tested without inducing necrosis. These properties may make the use of glufosinate advantageous in a selective growth system for detection of transformed coffee tissues. |
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Keywords: | callus induction Coffea spp. embryogenic callus suspensions genetic transformation somatic embryogenesis selective agents |
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