Evaluation of genotoxic and oxidative stress response to dimethoate in freshwater fish Channa punctatus (Bloch) |
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Authors: | Daoud Ali Phool Gend Kumar Sudhir Kumar Mukhtar Ahmed |
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Affiliation: | 1. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;2. E-mail: daudali.ksu12@yahoo.com;3. E-mail: aalidaoud@ksu.edu.sa;4. Molecular and Human Genetic Laboratory, Department of Zoology, University of Lucknow, Lucknow 226007 India |
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Abstract: | AbstractDimethoate is one of the organophosphate insecticides widely used in agriculture throughout the world and is an inhibitor of cholinesterase in animals. The objective of the present study was to detect oxidative stress and DNA damage induced by dimethoate in the freshwater fish Channa punctatus (C. punctatus). The LC50-96 h value of technical grade dimethoate was estimated at 19.10 μg L-1 in a semi-static system and, on the basis of the LC50 value, three concentrations were determined. The fish were exposed to these concentrations of dimethoate for 96 h and samplings were done at 24 and 96 h for assessment of oxidative stress and DNA damage. After exposure to dimethoate, the level of superoxide dismutase declined while lipid peroxidation, glutathione, induction of micronucleus and DNA damage were increased in C. punctatus as the concentration and exposure time increased. Thus our results suggest that dimethoate induces genotoxic effects which invariably accompanied and correlated with oxidative stress. |
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Keywords: | Channa punctatus dimethoate DNA damage comet assay oxidative stress |
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