Purification of recombinant proteins by immunoaffinity chromatography with preselected antibodies |
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| Authors: | Jytte Pedersen Claus Emborg Jane Sørensen Kirsten Biedermann |
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| Institution: | (1) Department of Biotechnology, The Technical University of Denmark, Building 223, DK-2800 Lyngby, Denmark |
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| Abstract: | Summary Nuclease produced by recombinant Escherichia coli was successfully purified by immunoaffinity chromatography using an antibody preselected for the purpose on the basis of an elution assay. The elution assay was also used to optimize elution conditions. One step recovery of nuclease from crude periplasmic extract was 66%. |
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