The binding of cytochalasin D to monomeric actin |
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| Authors: | D W Goddette C Frieden |
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| Institution: | 1. UFZ – Helmholtz Centre for Environmental Research, Department of Community Ecology, 06120 Halle, Germany;2. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, 04103 Leipzig, Germany;3. Université catholique de Louvain, Earth and Life Institute, 1348 Louvain-la-Neuve, Belgium;4. InForest JRU (CTFC-CREAF), 25280 Solsona, Spain;5. CREAF, 08193 Cerdanyola del Vallès, Spain;6. CSIC, 08193 Cerdanyola del Vallès, Spain;7. Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Baños, College, Laguna, 4031 Philippines |
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| Abstract: | The binding of cytochalasin D to monomeric actin has been measured directly. In the presence of 200 microM Ca2+, actin binds cytochalasin D in a 1:1 molar ratio with a KD of 18 microM. After incubation with 250 microM Mg2+ for 10 minutes, actin binds cytochalasin D with a KD of 2.6 microM but with one mole of cytochalasin D per 2 moles of actin. This suggests that cytochalasin D induces dimerization of Mg2+-induced actin monomers. |
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