Near on-line detection of enteric bacteria using lux recombinant bacteriophage |
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Authors: | Chandra P Kodikara Hilary H Crew Gordon SAB Stewart |
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Institution: | University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington, U.K. |
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Abstract: | The potential for a revolution in microbial testing can be perceived with the near on-line detection of indicator microorganisms. By definition, these are microorganisms present in significant numbers within a food which, while not pathogenic, can be related through increasing count to the increased probability of pathogen contamination. We have used recombinant lux+ bacteriophage to detect enteric indicator bacteria without recovery or enrichment in 50 min, provided that they are present at levels greater than 10(4) g-1 or cm-2. After a 4-h enrichment, samples having enteric counts of 10 g-1 or cm-2 can be distinguished from background. |
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Keywords: | Bioluminescence Bacteriophage Enteric bacteria Bacterial detection |
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