Possible role of PEPT1 in gastrointestinal hormone secretion |
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Authors: | Matsumura Kimio Miki Takashi Jhomori Takahito Gonoi Tohru Seino Susumu |
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Affiliation: | Division of Cellular and Molecular Medicine, Kobe University Graduate School of Medicine, Japan. |
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Abstract: | Oligopeptides originating from ingested meal stimulate the secretion of various gastrointestinal hormones, but the mechanism is unknown. In this study, we show that transfection of oligopeptide transporter 1 (PEPT1) in STC-1 cells, a murine enteroendocrine cell line, evokes di-peptide-stimulated hormone secretion in a pH-dependent manner. Measurement of membrane potentials shows that PEPT1- transfected STC-1 cells are depolarized by di-peptide glycyl-glycine but not by glycine monomer. Glycyl-glycine stimulation induces a rise in the intracellular calcium concentration in PEPT1-transfected STC-1 cells. The secretion induced by glycyl-glycine in PEPT1-transfected STC-1 cells was blocked by nifedipine, a Ca(2+) channel blocker, suggesting that the secretion is triggered by Ca(2+) influx through L-type voltage-dependent Ca(2+) channels. These data suggest that PEPT1 mediates oligopeptide-induced hormone secretion in enteroendocrine cells. |
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Keywords: | STC-1 cells Enteroendocrine cells PEPT1 Oligopeptide sensing Hormone secretion |
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