Zinc L-carnosine protects colonic mucosal injury through induction of heat shock protein 72 and suppression of NF-kappaB activation |
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Authors: | Odashima Masaru Otaka Michiro Jin Mario Wada Isao Horikawa Youhei Matsuhashi Tamotsu Ohba Reina Hatakeyama Natsumi Oyake Jinko Watanabe Sumio |
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Institution: | Department of Gastroenterology, Akita University School of Medicine, 1-1-1, Hondo, Akita City, Akita 010-8543, Japan. odashima@doc.med.akita-u.ac.jp |
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Abstract: | In this study, we investigated the effects of zinc L-carnosine, an anti-ulcer drug, on acetic acid-induced colonic mucosal injury and the correlation of these effects with expression of 72-kDa heat shock proteins (HSP72) and nuclear factor kappa B (NF-kappaB) activation in rat colonic mucosa in vivo. After intrarectal administration of zinc L-carnosine, the rats received intrarectal infusion of 5% acetic acid (1 ml). The colonic mucosal damage was evaluated by macroscopic assessments 24 h after the intrarectal infusion of acetic acid. Expression of HSP72 in rat colonic mucosa was evaluated by Western blot analysis before and after zinc L-carnosine administration. NF-kappaB activation was evaluated by electrophoretic mobility shift assays (EMSA). Zinc L-carnosine inhibited visible damage in rat colonic mucosa by acetic acid. Expression of HSP72 was significantly increased at 6 h after zinc L-carnosine administration. Furthermore, NF-kappaB activation in colonic mucosa was suppressed 6 h after zinc L-carnosine treatment. These results suggested that zinc L-carnosine protects the colonic mucosa against acetic acid by induction of HSP72 and suppression of NF-kappaB activation and zinc L-carnosine may be a novel therapeutic agent for the therapy of inflammatory bowel disease. |
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Keywords: | l-carnosine" target="_blank">Zinc l-carnosine Cytoprotection HSP72 NF-κB |
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