Cytotoxic,immunosuppressive, trypanocidal and antileishmanial activities of Basidiomycota fungi present in Atlantic Rainforest in Brazil |
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Authors: | Luiz H Rosa Kátia M G Machado Ana L T Rabello Elaine M Souza-Fagundes Rodrigo Correa-Oliveira Carlos A Rosa Carlos L Zani |
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Institution: | 1.Laboratório de Microbiologia, Instituto de Ciências Exatas e Biológicas,Universidade Federal de Ouro Preto,Ouro Preto,Brazil;2.Universidade Católica de Santos,Vila Mathias,Brazil;3.Laboratório de Pesquisas Clínicas,Centro de Pesquisas René Rachou, Funda??o Oswaldo Cruz,Belo Horizonte,Brazil;4.Departamento de Fisiologia e Biofísica,Universidade Federal de Minas Gerais,Belo Horizonte,Brazil;5.Laboratório de Imunologia Celular e Molecular,Centro de Pesquisas René Rachou, Funda??o Oswaldo Cruz,Belo Horizonte,Brazil;6.Departamento de Microbiologia, Instituto de Ciências Biológicas,Universidade Federal de Minas Gerais,Belo Horizonte,Brazil;7.Laboratório de Química de Produtos Naturais,Centro de Pesquisas René Rachou, Funda??o Oswaldo Cruz,Belo Horizonte,Brazil |
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Abstract: | One hundred three Basidiomycota fungi representing 84 species and 17 families were collected from different Atlantic Rainforest
in Brazil. Their basidiomes and fermentation broth extracts were screened in a bioassay panel that included three human cancer
cells lines, human peripheral blood mononuclear cells (PBMCs), the enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, and amastigote forms of Leishmania amazonensis. Forty-two extracts representing 21 genera and 35 species presented activities higher than 60% in one or more assays employed
in this study. Eighteen extracts were toxic to one or more human cancer cell lines. Extracts from Lentinus
strigosus CCB 178 and Lentinus sp. UFMGCB 38 showed selectivity towards cancer cells as they showed only a minor impact on PBMCs. Six extracts suppressed
PBMCs proliferation and showed low toxic effect to cancer cells. Thirty-four extracts inhibited the activity of the TryR.
Of these, five showed low toxicity towards PBMCs. Extracts from Gymnopilus areolatus, Irpex lacteus, L.
strigosus, Nothopanus hygrophanus, Pleurotus flabellatus, and unidentified Basidiomycetes were toxic to L. amazonensis. The results of this screening reinforce the potential of Basidiomycota fungi as sources of bioactive natural products that
may be developed into new therapeutic agents for cancer and neglected diseases such as trypanosomiasis and leishmaniasis. |
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Keywords: | Fungi Cancer cells Immune system Natural products Leishmania Trypanothione reductase |
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