Production of sepiapterin in Escherichia coli by coexpression of cyanobacterial GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase |
| |
Authors: | Woo Hyun Joo Kang Jee Yun Choi Yong Kee Park Young Shik |
| |
Affiliation: | Department of Microbiology, Inje University, Kimhae 621-749, Korea. |
| |
Abstract: | Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|