Development and polymorphism of microsatellite markers for Fagus crenata and the closely related species, F. japonica |
| |
Authors: | K Tanaka Y Tsumura T Nakamura |
| |
Institution: | (1) Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan, JP;(2) Bio-resources Technology Division, Forestry and Forest Products Research Institute, Kukizaki, Ibaraki 305-8687, Japan, JP |
| |
Abstract: | We have developed microsatellite markers (SSRs) applicable to Fagus crenata using the RAHM method and investigated their polymorphisms. We also applied the SSRs in an analysis of a closely related
species, F. japonica. Here we describe the isolation and characterization of nine polymorphic microsatellite markers, of which eight are applicable
to both species. Among 30 individuals of each of F. crenata and F. japonica we detected a total of 79 and 77 alleles, respectively, with an average of 9.9 and 8.6 alleles per locus. The mean expected
heterozygosity (He) was 0.615 (range: 0.216–0.925) in F. crenata and 0.660 in F. japonica (range: 0.259–0.827). The He values were considerably higher than those previously found for isozymes. Paternity exclusion probabilities for multiple
loci, calculated over all loci, were extremely high (0.999 and 0.998 in F. crenata and F. japonica, respectively): sufficiently high to study pollen flow in both species.
Received: 5 December 1998 / Accepted: 28 December 1998 |
| |
Keywords: | Fagus crenata Fagus japonica Microsatellite RAPD RAHM SSR |
本文献已被 SpringerLink 等数据库收录! |
|