Comparison of genomic SSR and EST-SSR markers for estimating genetic diversity in cucumber |
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Authors: | J Hu L Wang and J Li |
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Institution: | (1) College of Horticulture, Henan Agricultural University, Zhengzhou, 450002, China;(2) College of Horticulture, Northeast Agricultural University, Harbin, 150030, China |
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Abstract: | Thirteen genomic microsatellite (gSSR) and sixteen expressed sequence tag (EST)-SSR (eSSR) markers were compared to estimate
genetic diversity among 29 cucumber (Cucumis sativus L.) accessions. gSSR markers detected mean 4.46 alleles with a mean polymorphic information content (PIC) of 0.664, against
eSSR markers with mean 3.38 alleles and a mean PIC of 0.397. gSSRs amplified more null alleles than eSSRs. Genetic diversity
within the accession set was estimated by construction of dendrograms using gSSR or eSSR data. There was a clear consistency
between gSSR and eSSR trees in terms of positioning of most cucumber germplasms. gSSR markers could separate various types
of cucumber germplasms on the whole, although clustering of some accessions was not based on their geographical origins in
eSSR tree. eSSR markers identified an independent sub-cluster containing five accessions resistant to downy mildew, suggesting
a probable relationship between eSSRs and disease-resistance trait in cucumber. The Mantel test between gSSR and eSSR matrices
revealed a good fit correlation (r = 0.836). The general dendrogram constructed using the combined data of gSSRs and eSSRs was similar to those obtained separately
with each marker. |
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