Optimization of serum free medium for cord blood mesenchymal stem cells |
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| Affiliation: | 1. Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan, Taiwan;2. Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan;1. University of Trieste, Department of Engineering and Architecture, Via A. Valerio 10, 34127, Trieste, Italy;2. Wrocław University of Science and Technology, Department of Biomedical Engineering, Mechatronics and Theory of Mechanisms, Łukasiewicza 7/9, 50-371 Wrocław, Poland;1. Center for Stem Cell&Organoid Medicine (CuSTOM), Perinatal Institute, Divisions of Developmental Biology the Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA;2. Pulmonary Biology, Cincinnati Children''s Hospital, and the Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA;1. Department of Plastic Surgery, Shenzhen Qianhai Taikang Hospital, Shenzhen, 518000, Guangdong, China;2. Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, China |
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| Abstract: | Human umbilical cord blood harbors mesenchymal stem cells (MSCs), which can give rise to several mesenchymal lineages. In order to explore their usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. Additionally, the development of a serum-free medium becomes indispensable for elimination of possible contaminants from the serum-containing medium during expansion. Using fractional factorial designs combined with the steepest ascent approach, we have developed a serum-free medium that could ex vivo expand MSCs over nine passages, resulting in at least 1000-fold increases in cell number within 1-month. Based on Iscove's modified Dulbecco's medium, this medium formulation includes bFGF (17.91 ng/mL), human albumin (2.80 mg/mL), hydrocortisone (27.65 μM) and SITE (1.18%, v/v). The expanded MSCs in the designed medium preserved differentiation potentials into three mesenchymal lineages in vitro, including chondrocytes, adipocytes and osteoblasts. In conclusion, we optimized a serum-free and defined culture medium for cord blood-derived MSCs, which could be applied to cell-based therapy and biomedical research. |
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