Knockout of cGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells |
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| Authors: | Martijn A Langereis Huib H Rabouw Melle Holwerda Linda J Visser Frank J M van Kuppeveld |
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| Institution: | Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands |
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| Abstract: | It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells. |
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