Catalytic properties of cross-linked enzyme crystals in organic media |
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| Institution: | 1. Department of Culinary Arts, Chung Chou University of Science and Technology, Changhua 510, Taiwan;2. Department of Food Science, Tunghai University, Taichung 407, Taiwan;3. Department of Medicinal Botanicals and Health Applications, Da-Yeh University, Changhua 515, Taiwan |
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| Abstract: | The activity and the enantioselectivity of cross-linked enzyme crystals (CLECs) of subtilisin in the transesterification between N-acetyl-l-phenylalanine ethyl ester and n-propanol have been examined in various organic solvents. The activity of CLECs of subtilisin in decane was 780 times greater than that in triethylamine. CLECs of subtilisin preferred l-enantiomer in the transesterification between N-acetyl-phenylalanine ethyl ester and n-propanol, and the (kcat/KM)L/(kcat/KM)D ratio was 20 000 in cyclohexane. |
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