Production of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. TS1-1: Optimization of carbon and nitrogen concentration in the feed medium using central composite design |
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| Institution: | 1. Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia;2. Department of Biology, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia;3. School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia;1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People''s Republic of China;2. Biotechnology Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, People''s Republic of China;3. CAAS-ICRAF Joint Laboratory on Agroforestry and Sustainable Animal Husbandry, Beijing 100193, People''s Republic of China;1. Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia;2. Comparative Genomics and Genetics Research Centre, Malaysia Genome Institute, Kajang, Selangor, Malaysia;3. School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia;1. Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 16100, FI-00076 Aalto, Finland;2. Polytech Clermont-Ferrand, School of Engineering, Department of Biological Engineering, BP 206, FR-63174 Aubière, France;3. University of Eastern Finland, Department of Chemistry, P.O. Box 111, FI-80101 Joensuu, Finland;1. TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France;2. Department of Chemistry, University of Copenhagen, Copenhagen, Denmark;1. Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing, China;2. College of Food Science and Nutritional Engineering, Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, China;1. FARE Laboratory, Chaire AFERE, Université de Reims Champagne-Ardenne, INRA, 51100 Reims, France;2. Plateforme de Modélisation Moléculaire Multi-échelle, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, 51687 Reims Cedex 2, France;3. Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, Université de Reims Champagne-Ardenne, 51687 Reims Cedex, France |
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| Abstract: | Optimisation of nutrient feeding was developed to overcome the limitation in batch fermentation and to increase the CGTase production from Bacillus sp. TS1-1 in fed batch fermentation. Optimisation of the C/N ratio in the feed stream was conducted in a 5 l fermenter, where feeding was initiated at constant rate of 0.02 h?1. In our initial screening process, the addition of nitrogen source boosted the growth of the microbes, but on the other hand reduced the CGTase production. The amount of tapioca starch and yeast extract was optimised in order to obtain a sufficient growth and thus, increased the CGTase production. Results were analysed using three-dimensional response surface plot, and the optimised values of carbon and nitrogen concentration of 3.30% (w/v) and 0.13% (w/v) were obtained, respectively. CGTase activity increased up to 80.12 U/ml, which is 13.94% higher as compared to batch fermentation (70.32 U/ml). This also led to 14.54% increment of CGTase production in fed batch culture as compared to the production before the optimisation. The CGTase activity obtained was close to the predicted value, which is 78.05 U/ml. |
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