Improved accumulation of phenylethanoid glycosides by precursor feeding to suspension culture of Cistanche salsa |
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| Institution: | 1. College of Life Sciences, Northwest A & F University, No. 22, Xinong Rd, Yangling 712100, China;2. Tianjin Tasly Modern Traditional Chinese Medicine Resources Co., Ltd., Tasly Modern TCM Garden, Beichen Hi-Tech Park, Tianjin 300402, China;3. Hanzhong Institute of Botany, Jiangtan West Rd, Hanzhong 723000, China;1. Division of Infectious Diseases & HIV Medicine, University Hospitals Case Medical Center, 11100 Euclid Avenue, Cleveland, OH 44106, USA;2. Department of Medicine, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA;3. Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 10701 East Boulevard, Cleveland, OH 44106, USA;4. Geriatrics Research, Education and Clinical Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 10701 East Boulevard, Cleveland, OH 44106, USA;5. Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA;6. Department of Pharmacology, Case Western Reserve University School of Medicine, 10900 Euclid Avenue, Cleveland, OH 44106, USA;1. Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151, Lodz, Poland;2. Department of Pharmacognosy, Medical University of Lodz, Muszynskiego 1, 90-151, Lodz, Poland;3. Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097, Warsaw, Poland;1. Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, 51000 Rijeka, Croatia;2. Medical Faculty, University of Rijeka, 51000 Rijeka, Croatia;3. Department of Endocrinology, Clinical Hospital Rijeka, 51000 Rijeka, Croatia;1. Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, PO-CIMAP, Lucknow, 226015, India;2. Molecular Bioprospection Division, Central Institute of Medicinal & Aromatic Plants, Council of Scientific & Industrial, Research, PO- CIMAP, Lucknow, 226015, India;1. Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland;2. Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland |
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| Abstract: | Effects of some precursors on phenylethanoid glycosides (PeGs) accumulation in Cistanche salsa cell suspension cultures were investigated. Precursors such as tyrosine, phenylalanine, caffeic acid and cucumber juice at proper concentrations could increase the total accumulation of PeGs (echinacoside, acteoside, 2′-acetylacteoside) by 50%, 12%, 12% and 23%, respectively. Under the combined feeding of precursors at proper concentrations, the total production of PeGs in bio-staged culture reached the highest amount of 1358.1 mg l?1 (640.8 mg echinacoside l?1, 689.4 mg acteoside l?1 and 54.9 mg 2′-acetylacteoside l?1), which was about two-fold of that in the control. This study showed promise for obtaining large-scale production of active ingredients in plant cells by the solid–liquid two step culture (SLTSC) technique and also provided for the first time an example for producing PeGs by C. salsa cell culture. The improved production of PeGs was higher than that in previous reports on PeG production by Cistanche deserticola cell culture fed with precursors. |
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