Optimization of polysaccharide and ergosterol production from Agaricus brasiliensis by fermentation process |
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| Institution: | 1. Department of Microbiology and Parasitology, China Medical University, No.77 Puhe Road, Shenyang 110122, Liaoning Province, China;2. National Key Laboratory of Biochemical Engineering, National Engineering Research Center for Biotechnology (Beijing), Key Laboratory of Biopharmaceutical Production & Formulation Engineering, PLA, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 North 2nd Street, Beijing 100190, China;1. Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands;2. CNRS, Aix-Marseille Université, Marseille, France;3. INRA, USC 1408 AFMB, 13288 Marseille, France;4. Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia;5. Department of Food and Environmental Sciences, University of Helsinki, Viikinkaari 9, Helsinki, Finland |
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| Abstract: | Statistically based experimental designs were applied for the fermentation optimization of polysaccharide and ergosterol production from Agaricus brasiliensis in a stirred-fermenter. Culture temperature, agitation speed and initial pH were identified to have significant effects on polysaccharide and ergosterol production by a Plackett–Burman design. These three significant factors were subsequently optimized using steepest ascent method and Box–Behnken design. The validity of the optimum conditions was verified by separate experiments in which the polysaccharide and ergosterol yields were increased by 57% and 43%, respectively, as compared to those under unoptimized fermentation conditions. |
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