FM1-43 measurements of local exocytotic events in rat melanotrophs |
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Authors: | Sikdar S K Kreft M Pangrsic T Grilc S Zorec R |
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Institution: | Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, Medical Faculty, University of Ljubljana Medical School, Zaloska 4, SI-1000 Ljubljana, Slovenia. |
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Abstract: | We have explored the existence of fusion- and secretion-competent sites on the plasma membrane of peptide secreting rat pituitary melanotrophs at rest, and following stimulation with glutamate. We monitored changes in fluorescence of FM1-43, a styryl dye which labels plasma membrane. The results show spontaneous local increases in FM1-43 reporting changes in membrane surface area due to cumulative exocytosis. Addition of glutamate, further increased the occurrence of these events. Statistical analysis of local FM1-43 fluorescence changes suggests that this is due to the recruitment of inactive exocytotic domains and due to the stimulation of already active exocytotic domains. |
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Keywords: | Neuroendocrine cell Exocytosis FM1-43 Hormone secretion Exocytotic domain |
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