Small repeated sequences in the chloroplast genome of Chlamydomonas reinhardi |
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| Authors: | Stanton B Gelvin and Stephen H Howell |
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| Institution: | (1) Department of Biology, University of California, San Diego, 92093 La Jolla, California, USA;(2) Present address: Department of Microbiology and Immunology, School of Medicine, University of Washington, 98195 Seattle, Washington, USA |
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| Abstract: | Summary Chloroplast DNA of Chlamydomonas reinhardi contains many inverted repeated sequences. Analysis by hydroxyapatite binding, S1 nuclease digestion, and electron microscopy indicates that these sequences are 0.1–0.3 kilobase pairs in length, are widely distributed in the chloroplast genome, and make up 4–7% of the chloroplast DNA.Abbreviations RNA
ribonucleic acid
- rRNA
ribosomal RNA
- RNA
complementary RNA
- DNA
deoxyribonucleic acid
- chl DNA
chloroplast DNA
- HAP
hydroxypatite
- SSC
0.15 M NaCl, 0.015 M sodium citrate
- 0.1xSSC, 2xSSC, 4.67xSSC
0.1, 2, and 4.67 times the concentration of SSC, respectively
- TCA
trichloroacetic acid
- PB
NaPO4 buffer, pH 6.8
- Kb
Kilobase
- KbP
Kilobase pair |
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| Keywords: | |
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