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Aeration alleviates ethanol inhibition and glycerol production during fed-batch ethanol fermentation |
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| Authors: | Hyeon-Beom Seo Ji-Hyeon Yeon Myung Hoon Jeong Do Hyung Kang Hyeon-Yong Lee Kyung-Hwan Jung |
| Institution: | 1. Department of Biotechnology, Chungju National University, Chungbuk, 368-701, Korea 2. Division of Biomaterials Engineering, Kangwon National University, Chuncheon, 200-701, Korea 4. Korea Ocean Research & Development Institute, Ansan, 426-744, Korea 3. Research Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea |
| Abstract: | In this study, we investigated the effects of aeration on ethanol inhibition and glycerol production during fed-batch ethanol fermentation. When aeration was conducted at 0.13, 0.33, and 0.8 vvm, the ethanol productivity, specific ethanol production rate, and ethanol yield in the presence of greater than 100 g/L of ethanol were higher than when aeration was not conducted. In addition, estimation of the parameters (α and β) in a model equation of ethanol inhibition kinetics indicated that aeration alleviated ethanol inhibition against the specific growth rate and the specific ethanol production rate. Specifically, when aeration was conducted, the glycerol yield and specific glycerol production rate decreased approximately 50 and 70%, respectively. Finally, the results of this study indicated that aeration during fed-batch ethanol fermentation may improve the ethanol concentration in the final culture broth, as well as the ethanol productivity. |
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