A HPTLC method for the determination of the mycotoxin zearalenone in cereal products |
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Authors: | Email author" target="_blank">V?OstryEmail author J?Skarkova |
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Institution: | (1) National Institute of Public Health Prague, Centre for the Hygiene of Food Chains in Brno, National Reference Centre for Microscopic Fungi and Mycotoxins in Food Chains, The Czech Republic |
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Abstract: | An HPTLC method for the quantification of zearalenone (ZEA) in cereals and cereal products (wheat flour and malt) has been
developed. ZEA was extracted with 50 ml of acetonitrile-purified water (9+1) with addition of 2 g NaCl. The extracts were
further purified on VICAM ZearalaTest(tm) immunoaffinity columns, then analysed by instrumental high-performance thin-layer chromatography (HPTLC) on silica gel plates
with fluorescence detection. Ethyl acetate - n-hexane (1+1) was used as the mobile phase. The chromatogram was scanned in
fluorescence mode after excitation at λ=254 nm with λ=400 nm measuring filter: SENS and SPAN parameters were 195 and 20, respectively.
TheR
F of ZEA under these conditions was 0.43.
The recovery was 95% in the range 15–65 μg/kg cereal products; the mean relative standard deviation of repeatability (RSDr)
was 7.6%. The limit of quantification (LoQ) of ZEA was 10 μg/kg. Validation of the method was performed according to the principles
of the ICH for pharmaceutical analysis.
Presented at the 25th Mykotoxin Workshop in Giessen, Germany, May 19–21, 2003 |
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Keywords: | zearalenone cereal products quantitative analysis immunoaffinity columns instrumental HPTLC |
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