微生物表面展示技术及其在环境污染治理中的应用

微生物表面展示技术是通过基因工程手段，将短的外源肽或蛋白质表达在微生物细胞表面，该技术可以应用于开发活的细菌疫苗、筛选抗体库、生产生物细胞吸附剂以及制备整细胞生物催化剂。通过金属高效结合肽的肽库筛选和微生物展示技术，将金属结合肽直接展示在微生物的表面，用于处理环境中的重金属污染，为环境中重金属污染的防治提供了一条崭新的途径。利用微生物表面展示技术制备整细胞催化剂，用于有毒有机污染物的处理，可以极大地加快污染物的降解速率。简要介绍了微生物表面展示技术及其在重金属污染治理和毒性有机污染物的脱毒等环境生物修复方面的最新研究进展。

Microbial Cell-surface Display and the Application in Bioremediation of Contaminated Environment

Microbial cell-surface display technique allows peptides and proteins to be displayed on the surface of microbial cells. The expression of proteins on the surface of microbial cells has proved extremely important for numerous applications, including live vaccine development, peptide library screening, detoxification of harmful chemicals and biosorption of heavy metals. The principle of cell-surface display and the application in environmental bioremediation purposes, such as biosorbents for heavy metal removal and whole cell biocatalysts for detoxification of organic contaminants was briefly introduced.