雅氏瓦螨对氟胺氰菊酯的抗性机理初探

用有效成分为1000μg/L的氟胺氰菊酯药液对雅氏瓦螨Varroa jacobsoniOudemans敏感个体的腹部表皮进行穿透性测定,结果表明药液无法通过表皮起毒杀作用。通过添加酶抑制剂多功能氧化酶(PBO)和酯酶(DEF)的增效测定,结果显示PBO在抗性和敏感螨中分别增加毒效为3.27和1.80倍;而DEF为3.23和1.67倍。反映在抗性蜂螨的抗药性与多功能氧化酶的活性有密切相关,也与酯酶活性有关。对抗性螨和敏感螨的羧酸酯酶的活力测定,显示出在抗性螨中酶活指数高140%以上。同时对酯酶电泳进行扫描,也发现抗性螨与敏感螨的峰值存在差异。

Preliminary research on mechanism of fluvalinate resistance in Varroa jacobsoni

Mechanisms of fluvalinate resistance in the mite Varroa jacobsoni Oudemans, a parasite of honeybees, were tested.PBO and DEF had significant synergism to fluvalinate in the resistant and susceptible mites, and the synergism ratio was 3.23 and 1.67 fold in PBO, was 3.27 and 1.80 fold in DEF, respectively. The results indicate that higher activities of mixed function oxidase and esterase were found in the resistant strain. The carboxylesterase activity was 140% higher in resistant mites relative to the susceptible mites. Esterase electrophoresis showed that there were several differences between resistant and susceptible mites.