Effects of taurine on polymorphonuclear phagocytosis activity in burned patients |
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Authors: | Farriol M Venereo Y Rosselló J Gomez P Palao R Orta X Segovia-Silvestre T |
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Institution: | (1) Centre d'Investigacions Bioquímicas i Biología Molecular (CIBBIM),, ES;(2) Servei de Medecina Preventiva, and, ES;(3) Servei de Grands Cremats, Hospital Vall d'Hebron, Barcelona, Spain, ES |
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Abstract: | Summary. This study determines the effects of taurine (Tau) on phagocytosis of polymorphonuclear neutrophils (PMN) isolated from normal
subjects (n = 41) and severely burned patients (n = 20). Phagocytosis was measured by nitroblue of tetrazolium (NBT) reduction
in samples with and without latex bead stimulation. Taurine was added at doses of 0.2, 0.4, 0.8 and 1.6 mM to stimulated samples.
In control cells there were statistically significant increases in phagocytosis after addition of Tau 0.8 mM and 1.6 mM to
as compared to samples without Tau addition (295 ± 23% and 330 ± 35% vs. 248 ± 18%; mean ± S.E.; p < 0.05). A statistically
significant increase in phagocytosis was observed in cells from the burned population after addition of Tau 1.6 mM (288 ±
38% vs. 198 ± 13%; mean ± S.E.; p < 0.05). No changes in phagocytosis were found in cells from a subgroup of burn patients
(n = 13) followed over 7, 15 and 21 days. These results indicate that taurine supplementation in vitro at doses of 0.8 to
1.6 mM improves the phagocytic capacity of neutrophils in healthy subjects and in patients with severe burn injury, mainly
when neutrophil function is unaltered.
Received December 17, 2001 Accepted January 17, 2002 Published online August 30, 2002
Authors' address: Dr. Mireia Farriol, Centre d'Investigacions Bioquímicas i Biología Molecular (CIBBIM), Hospital General Vall d'Hebron Passeig
Vall d'Hebron 119-129, E-08035 Barcelona, Spain, Fax: 34-93-2746831, E-mail: farriol@hg.vhebron.es |
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Keywords: | : Phagocytosis Taurine Neutrophils NBT |
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