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Photosynthesis-Dependent Volume Regulation in Guard Cell Protoplasts from Vicia faba L. |
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| Authors: | Gotow Kiyoshi; Shimazaki Ken-ichiro; Kondo Noriaki; Syono Kunihiko |
| Institution: | 1Plant Physiology, Tokyo Gakugei University Tokyo 184, Japan 2Division of Environmental Biology, The National Institute for Environmental Studies Yatabe machi, Tsukuba, Ibaraki 305, Japan 3Department of Pure and Applied Sciences, College of General Education, University of Tokyo Tokyo 153, Japan |
| Abstract: | A rapid and convenient procedure was developed for isolatingguard cell protoplasts (GCPs) from epidermal strips of Viciafaba L. The mean rates of O2 uptake in the dark and evolutionin light of the isolated GCPs were 200 and 290 µmol O2mg–1 Chl h–1, respectively, showing net O2 evolutionin light. Photosynthetic O2 evolution was suppressed completelyby 5 µM DCMU. Addition of 5 µM DCMU to the incubationmedium after 30 min of light exposure also suppressed the light-inducedswelling of GCP, indicating possible participation of PS IIin volume regulation in GCP. 4Present address: Division of Environmental Biology, The NationalInstitute for Environmental Studies, Yatabe machi, Tsukuba,Ibaraki 305, Japan. (Received December 17, 1983; Accepted March 21, 1984) |
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