Stable synthesis of viral protein 2 of infectious bursal disease virus in Saccharomyces cerevisiae. |
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| Authors: | M N Jagadish D L Laughton A A Azad I G Macreadie |
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| Institution: | CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, Victoria, Australia. |
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| Abstract: | Viral protein 2 (VP2) from infectious bursal disease virus and its precursor polyprotein (N-VP2-VP4-VP3-C), in the absence of their native N-terminal region (19 amino acids), required fusion of yeast presequences for their stable synthesis in Saccharomyces cerevisiae Jagadish et al., Gene 95 (1990) 179-186]. Restoration of the missing 19 aa resulted in stable synthesis of VP2, indicating the significance of the N-terminal region in protein stability. |
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