Soybean leghemoglobin targeted to potato chloroplasts influences growth and development of transgenic plants |
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| Authors: | A. Chaparro-Giraldo R. M. Barata S. M. Chabregas R. A. Azevedo M. C. Silva-Filho |
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| Affiliation: | Departamento de Genética, Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de S?o Paulo, Av. Pádua Dias,11, Caixa Postal 83, 13400–970 Piracicaba, SP, Brazil e-mail: mdcsilva@carpa.ciagri.usp.br Fax: +55-19-4336706, BR
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| Abstract: | Potato tubers were transformed with a chimeric gene made by the fusion of the soybean leghemoglobin encoding gene (lba) with the chloroplastic targeting sequence from Rubisco. This construct was placed under the control of the strong constitutive 35S promoter and the 3′ nontranslated region of Rubisco from pea. Leghemoglobin expression on kanamycin-resistant plants was monitored by RT-PCR. Furthermore, immunodetection of subcellular fractions of transgenic plants revealed that leghemoglobin was imported and correctively processed inside the organelle. In addition, analysis of transgenic plants revealed reduced growth and decreased tuber production compared with the untransformed plants. It is suggested that leghemoglobin expression in potato chloroplasts interferes with aerobic metabolism, leading to physiological and morphological changes. Received: 20 December 1999 / Revision received: 28 May 2000 / Accepted: 16 June 2000 |
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| Keywords: | Leghemoglobin Potato Aerobic metabolism |
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