Use of impedance measurements to estimate numbers of antibiotic resistant Salmonella strains |
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Authors: | Angela M.,Gibson &dagger |
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Affiliation: | Agricultural and Food Research Council, Institute of Food Research—Bristol Laboratory, Langford, Bristol BS18 7DY |
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Abstract: | Impedance detection times were compared with traditional plating methods for enumerating antibiotic-resistant strains of Salmonella stanley, Salm. thompson and Salm. infantis grown in laboratory medium and pork slurry. The correleation of log10 counts of salmonellas with detection times was highly significant ( r = -0·96 for broth and r = -0·94 for slurries. The confidence limits (± og10 1·0 for broth and ± log10 1·65 for slurry) indicated that detection times could reliably be used as a rapid means of enumerating salmonellas when large numbers of counts of known strains are required for growth studies. Use of antibiotic-resistant strains also permitted their selective detection by impedance from the natural spoilage flora of pork slurry when the same antibiotics were incorporated in the detection medium. |
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