A catalytic carbohydrate contributes to bacterial antibiotic resistance |
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| Authors: | Paul de Figueiredo Becky Terra Jasbir Kaur Anand Toshiyuki Hikita Martin Sadilek Dave E. Monks Anastasiya Lenskiy Senitiroh Hakomori Eugene W. Nester |
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| Affiliation: | (1) Department of Microbiology, University of Washington, Box 357242, Seattle, WA 98195, USA;(2) Present address: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA;(3) Present address: Department of Microbiology, Immunology, and Molecular Genetics, University of California, 4550 MSB, 609 Charles E. Young Dr. East, Los Angeles, CA 90095, USA;(4) Pacific Northwest Research Institute, 720 Broadway, Seattle, WA 98122, USA;(5) Present address: Departments of Biochemistry and Pediatrics, Teikyo University School of Medicine, Kaga 2-11-1, Itabashi-ku, Tokyo 173-8605, Japan;(6) Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195-1700, USA;(7) Department of Pathology, University of Washington, Box 357470, Seattle, WA 98195, USA |
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| Abstract: | Penicillins are widespread in nature and lethal to growing bacteria. Because of the severe threat posed by these antibiotics, bacteria have evolved a wide variety of strategies for combating them. Here, we describe one unusual strategy that involves the activity of a catalytic carbohydrate. We show that the cyclic oligosaccharide, β-cyclodextrin (βCD), can hydrolyze, and thereby inactivate, penicillin in vivo. Moreover, we demonstrate that this catalytic activity contributes to the antibiotic resistance of a bacterium that synthesizes this oligosaccharide in the laboratory. Taken together, these data not only expand our understanding of the biochemistry of penicillin resistance, but also provide the first demonstration of natural carbohydrate-mediated catalysis in a living system. Paul de Figueiredo, Becky Terra and Jasbir Kaur Anand have contributed equally to this work. |
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| Keywords: | Catalysis β -lactamase Alkaliphile Cyclodextrin Resistance |
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