Isolation and characterization of a sporeless mutant in Pleurotus eryngii |
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Affiliation: | 1. Institute for Food Technology and Biochemistry, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia;2. Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, 11001 Belgrade, Serbia;3. Institute of Oncology and Radiology of Serbia, Pasterova 14, 11000 Belgrade, Serbia;4. Institute of Agricultural Engineering, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia;5. Plant Research International, Wageningen University and Research Centre, Droevendaalsesteeg 1, Wageningen 6700 AA, The Netherlands |
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Abstract: | A sporeless mutant dikaryon, completely defective in sporulation, was isolated from mycelial protoplasts of Pleurotus eryngii mutagenized by UV irradiation. Newly established dikaryons between one component monokaryon from the mutant, and 12 different wild type monokaryons from 3 other wild type dikaryons, all exhibited the sporeless phenotype, whereas those between the other monokaryon and the same wild type monokaryons all produced normal fruiting bodies. These results indicated that the sporeless mutation was induced in one of two nuclei of the mutant and was dominant. In the wild type basidia, the pattern of nuclear behavior during sporulation corresponded to the pattern C nuclear behavior as defined by Duncan and Galbraith. Cytological observation revealed that in the sporeless mutant meiosis was blocked at the meta-anaphase I in most basidia and hence basidiospores and sterigmata were not produced. Although fruiting bodies of the sporeless mutant showed a somewhat leaning growth, their gross morphology and its fruiting body productivity were comparable to that of the original wild type strain. Based on these results, it was considered that the sporeless mutant could serve as a potential material in breeding of sporeless P. eryngii commercial strains. |
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Keywords: | Nuclear behavior Sporeless mutation |
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