Odorant-Binding Protein from Culex tarsalis,the Most Competent Vector of West Nile Virus in California |
| |
| Institution: | 1. Laboratoire Biodiversité et Environnement: Interaction Génome, Faculté des Sciences Biologique, Université des Sciences et de la Technologie Houari Boumediene, Alger 16111, Algeria;2. Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques (CRAPC), Zone Industrielle, BP 284 Bou-Ismail, Tipaza, Algeria;3. Laboratoire des Biotechnologies Liées à la Reproduction Animale, Institut des Sciences Vétérinaires, Université Blida 1, BP 270 Blida, Algeria;4. National Centre for Biotechnology Research, Ali Mendjli Nouvelle Ville, UV 03, BP E73 Constantine, Algeria;5. Veterinary and Agricultural Sciences Institute, Department of Veterinary Sciences, University of Batna 1, Batna, Algeria;6. Centre National de Référence des Francisella, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble Alpes, 38043 Grenoble, France;7. Centre National de la Recherche Scientifique, TIMC-IMAG, UMR5525, Université Grenoble Alpes, 38400, Saint Martin d''Heres, France;8. Ecole Supérieure des Sciences de l''Aliment et des Industries Alimentaires, Alger 16004, Algeria |
| |
| Abstract: | We have identified and cloned an odorantbinding protein from the female mosquito, Culex tarsalis (CtarOBP). As expected for an olfactory protein, CtarOBP was detected by gel electrophoresis analysis in antennae but not in control tissues (legs). The isolated protein was identified by in-gel digestion and subsequent analysis of internal fragments by tandem mass spectrometry (MS-MS). Based on the amino acid sequences of two peptides generated by enzymatic digestion, degenerate primers were designed for cDNA cloning. The complete cDNA (cloned by RACE) encoded a protein with a signal peptide (24 residues) and a mature protein of 125 amino acid residues. The calculated molecular mass and isoelectric point of the mature protein were 14,515 Da and pl 5.5, respectively. CtarOBP showed the hallmark of odorant-binding proteins, 6 cysteine residues, and high sequence homology (61–96%) to previously characterized mosquito OBPs. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
