思茅松毛虫肠道产脂肪酶菌株的筛选鉴定及酶学性质初步研究

利用纯培养和筛选培养,从思茅松毛虫幼虫肠道中分离得到7株产脂肪酶的菌株.通过提取基因组DNA并进行16S rDNA序列测定,构建产酶菌株的系统发育树,初步鉴定结果显示:菌株D2、D12、D19属于假单胞菌属(Pseudomonas sp.),菌株D7、D17属于芽胞杆菌属(Bacillus sp.),菌株D9、D16属于克雷伯氏菌(Klebsiella sp.).初步研究所产脂肪酶的酶学性质,确定这些酶的最适作用温度30～40℃、最适作用pH值8.0～9.0,为中温碱性脂肪酶.

Screening and Identification of Lipase-producing Bacteria from Intestinal Canal of Dendrolimus kikuchii and Preliminary Studies on Its Enzyme Properties

Seven lipase-producing bacterial strains(LPBS) were isolated from intestinal canal of larva of Kikuchi pine caterpillar(Dendrolimus kikuchii) using pure and screening culture.Through extraction of genomic DNA and measurement of 16S rDNA sequences,a phylogenetic tree of the LPBS was constructed.Initial characterization results showed that these strains belonged to Pseudomonas(D2,D12,D19),Bacillus(D7,D17),and Klebsiella(D9,D16).The enzyme properties of the produced lipase were preliminarily studied.And confirmed that the most suitable reaction conditions of the lipase was 30~40 ℃ and pH 8.0~9.0,they were mild alkaline lipase.