酯酶产生菌的分离与酶学性质研究

从餐馆附近下水道收集到的土壤中分离获得6株酯酶产生菌,其中S2菌株活性最高,从其形态特征、生理生化试验以及16S rRNA序列分析等方面,初步鉴定S2菌株为假单胞属(Pseudomonas sp.)。对该菌株的部分酶学性质进行了研究,发现该菌株所产的酯酶最适反应温度为40℃,最适反应pH为8.0;且该酯酶在温度为60℃以下和pH 7.0～10.0具有良好的稳定性。

Study on Isolation and Enzymatic Properties of Esterase-Producing Strain

Six esterase-produeing strains were isolated from the soil collected from the sewer near a restaurant, among them the esterase activity of strain S2 was the highest. It was initially identified as Pseudomonas sp. based on its mor- phological characteristics, physiological and biochemical tests and 165 rRNA gene sequence analysis. Parts of its en- zymatic properties were studied, the optimal temperature and pH of the esterase were 40℃ and 8.0 respectively. And the esterase had a good stability at temperatures below 60 ℃ and pH 7.0- ~ 10.0.