Location of a binding site for 1-anilinonaphthalene-8-sulfonate on calmodulin |
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| Authors: | R F Steiner |
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| Affiliation: | Department of Chemistry, University of Maryland Baltimore County, Catonsville, Maryland 21228 USA |
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| Abstract: | The quenching by radiationless energy transfer of the ultraviolet fluorescence of Tyr-99 and Tyr-138 by bound 1-anilinonaphthalene-8-sulfonate (1,8-ANS) has been employed to determine the separation of a hydrophobic binding site of 1,8-ANS from each of the tyrosines. The results suggest that the dominant binding site is located in the N-terminal region of domain III. |
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