Some morphogenic effects of sodium sulfate on tobacco callus |
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| Authors: | Stephen F Chandler Elena Ragolsky Eng-Chong Pua Trevor A Thorpe |
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| Institution: | (1) Plant Physiology Research Group, Department of Biology, University of Calgary, T2N 1N4 Calgary, Alberta, Canada;(2) Present address: Calgene Pacific Pty Ltd., P.O. Box 53, 3079 Ivanhoe, Victoria, Australia;(3) Present address: Institute of Molecular and Cell Biology, The National University of Singapore, Kent Ridge Road, 0511, Singapore |
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| Abstract: | Callus cultures of Nicotiana tabacum L cv. Wisconsin 38 were initiated and grown on shoot-forming (SF) and callus proliferation (CP) medium with or without Na2SO4. Two cultures were maintained on SF medium with 0, 0.75, 1 or 1.5% Na2SO4 for 2.5 and 3.5 years. In the older culture only callus grown on salt formed shoots throughout the maintenance period, while in the younger culture the control responded best and Na2SO4 was inhibitory. Callus from the older culture which had been grown on salt continued to form shoots in the absence of salt. Na2SO4 caused adventitious shoot formation in three cultures on CP medium. These shoots were present for 7 subcultures after removal of Na2SO4; but established, control callus, did not form shoots when transferred to Na2SO4. Callus initiated and maintained on NaCl or mannitol showed a slight increase in shoot initiation. On NaCl, Na2SO4 or mannitol, the tissue osmotic potential became more negative and proline concentration increased. |
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| Keywords: | callus cultures Nicotiana tabacum organogenesis sodium sulfate tobacco |
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