Limited variability of CTG/CAG repeats in <Emphasis Type="Italic">Lycopersicon</Emphasis> nuclear DNA |
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| Authors: | D Dimitrova O Georgiev C Valkova B Atanassova L Karagyozov |
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| Institution: | 1.Institute of Biophysics,Bulgarian Academy of Sciences,Sofia,Bulgaria;2.Institut für Molekularbiologie II,Universit?t Zurich,Zurich,Switzerland;3.Fritz Liepmann Institut für Altersforschung,Jena,Germany;4.Institute of Genetics,Bulgarian Academy of Sciences,Sofia,Bulgaria;5.Friedrich-Schiller Universit?t Jena,Jena,Germany |
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| Abstract: | Seven clones containing (CTG)n/(CAG)n repeats (n ≥ 4) were isolated by screening Lycopersicon esculentum genomic DNA. Four of the clones contained more than one simple sequence repeat (SSR). The SSRs were analyzed in several L. esculentum cultivars after polymerase chain reaction (PCR) amplification. No length variations were observed, suggesting considerable
locus stability. Five clones are from transcribed regions, which might explain the lack of cultivar variations. However the
conservation of CTG repeats was limited as differences in some transcribed loci were registered between L. pennellii and other Lycopersicon species. It is noted that in Lycopersicon trinucleotide repeat variation might be used for species identification. |
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| Keywords: | tomato transcribed DNA trinucleotide simple sequence repeats |
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