Characterization of technetium(vII) reduction by cell suspensions of thermophilic bacteria and archaea |
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| Authors: | Nikolay A Chernyh Sergei N Gavrilov Vladimir V Sorokin Konstantin E German Claire Sergeant Monique Simonoff Frank Robb Alexander I Slobodkin |
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| Institution: | (1) Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117312 Moscow, Russia;(2) Institute of Electrochemistry and Physical Chemistry, Russian Academy of Sciences, Leninskiy prospect 31, 119 991 Moscow, Russia;(3) Laboratoire de Chimie Nucleaire Analytique et Bioenvironnementale, CNRS-Universite de Bordeaux I, UMR 5084, Le Haut Vigneau, BP 120, 33175 Gradignan Cedex, France;(4) Center for Marine Biotechnology, 701 East Pratt Street, Baltimore, MD 21202, USA |
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| Abstract: | Washed cell suspensions of the anaerobic hyperthermophilic archaea Thermococcus pacificus and Thermoproteus uzoniensis and the anaerobic thermophilic gram-positive bacteria Thermoterrabacterium ferrireducens and Tepidibacter thalassicus reduced technetium 99Tc(VII)], supplied as soluble pertechnetate with molecular hydrogen as an electron donor, forming highly insoluble Tc(IV)-containing
grayish-black precipitate. Apart from molecular hydrogen, T. ferrireducens reduced Tc(VII) with lactate, glycerol, and yeast extract as electron donors, and T. thalassicus reduced it with peptone. Scanning electron microscopy and X-ray microanalysis of cell suspensions of T. ferrireducens showed the presence of Tc-containing particles attached to the surfaces of non-lysed cells. This is the first report on the
reduction in Tc(VII) by thermophilic microorganisms of the domain Bacteria and by archaea of the phylum Euryarchaeota. |
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| Keywords: | Technetium reduction Tc(VII) reduction Thermophilic microorganisms Radionuclide immobilization |
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