Isolation of histone-like proteins from mitochondria of bovine heart |
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| Authors: | E W Yamada H Dotzlaw N J Huzel |
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| Affiliation: | Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada. |
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| Abstract: | Two methods for isolating and purifying histone-like proteins from mitochondria of bovine heart are described. In the first, a sonicated extract of the mitochondria was fractionated in three chromatography steps, including affinity chromatography on DNA-cellulose, to purify a protein that resembles very closely the histone-like protein (HM) of yeast mitochondria. In the second method, an acid extract of the heart mitochondria was the starting material; two other histone-like proteins were separated. Thus, as in mitochondria of Xenopus laevis, several histone-like proteins are present in mitochondria of bovine heart. |
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