The effect of titanium with heparin/BMP-2 complex for improving osteoblast activity |
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| Authors: | Su-Young Lee Young-Pil Yun Hae-Ryong Song Heung Jae Chun Dae Hyeok Yang Kyeongsoon Park Sung Eun Kim |
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| Institution: | 1. Department of Prosthodontics, Seoul St. Mary''s Dental Hospital, The Catholic University of Korea, #505 Banpo-dong, Seocho-gu, Seoul 137-701, Republic of Korea;2. Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, #80 Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea;3. Institute of Cell & Tissue Engineering, Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, #505 Banpo-dong, Seocho-gu, Seoul 137-701, Republic of Korea;4. Division of Bioimaging, Chuncheon Center, Korea Basic Science Institute, 192-1 Hyoja 2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea |
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| Abstract: | The objective of this study was to investigate the enhanced osteoblast activity of MG-63 cells cultured on titanium (Ti) with a heparin/BMP-2 (Hep/BMP-2) complex. The Ti substrates were initially modified by chemical grafting poly-l-lysine (PLL) using condensing agent, followed by immobilizing the heparin/BMP-2 complex to the PLL-grafted Ti substrate via electrostatic interactions. The surface modification of Ti substrates with PLL and/or Hep/BMP-2 complex were confirmed with scanning electron microscopy, contact angle measurements, and X-ray photoelectron spectroscopy. Immobilized BMP-2 was released from the Hep/BMP-2/Ti substrate in a sustained manner. In vitro studies revealed that osteoblasts grown on Hep/BMP-2/Ti substrate increased ALP activity, calcium deposition, ALP and osteocalcin levels as compared to those grown on pristine Ti or PLL-Ti. These results indicated that heparin/BMP-2 complex immobilized Ti substrate can be useful to effectively improve osteoblast activity. |
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| Keywords: | Titanium Osteoblast activity Bone morphogenic protein-2 (BMP-2) Heparin |
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