Glucogalactan: A polysaccharide isolated from the cell-wall of Verticillium Lecanii |
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| Authors: | Maria L Corradi da Silva Diego TD Exposti Ana FD Vasconcelos Samara MA Alexandre Joana LM Silveira Diogo RB Ducatti |
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| Institution: | 1. Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CEP 19060-900, Presidente Prudente, São Paulo, Brazil;2. Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, Curitiba, Paraná, Brazil |
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| Abstract: | A water-soluble polysaccharide was extracted with alkali from the cell wall of Verticillium lecanii (also called Lecanicillium lecanii). After freezing and thawing, the water-soluble fraction was purified by gel filtration chromatography on Sepharose CL-6B and eluted as one peak by HPSEC/RID. Monosaccharide analysis showed galactose and glucose (1.1:1), with traces of mannose (<1%). The structural characteristics were determined by spectroscopic analysis, FT-IR and 1D and 2D 1H and 13C NMR, and methylation results. On the basis of the data obtained, the following structure of the polysaccharide (E3SIV fraction) was established: |
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| Keywords: | Verticillium lecanii Lecanicillium lecanii Glucogalactan NMR spectroscopy |
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