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Interaction of alkaloids with plant transfer ribonucleic acids. Effect of sparteine on lupin arginyl-tRNA formation |
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| Authors: | Tadeusz Zwierzyński Andrzej Joachimiak MirosŁawa Barciszewska Katarzyna Kulińska Jan Barciszewski |
| Institution: | Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61 704 Poznań Poland |
| Abstract: | The effect of the alkaloid sparteine on arginyl-tRNA formation was studied. It was demonstrated that sparteine sulfate in the concentration range 10–60 mM inhibits the charging reaction when amino acid, ATP and tRNA are used as variable substrates. The mode of action is different for all substrates studied. It was concluded that at high sparteine concentration the pattern of inhibition for all varied substrates is generally uncompetitive. A non-competitive mechanism for amino acid and tRNA was observed at low sparteine concentration, but in the case of ATP it is also uncompetitive. |
| Keywords: | Alkaloids Sparteine Plant tRNA Aminoacylation of tRNA Aminoacyl tRNA synthetase Arginyl-tRNA synthetase AA-tRNA aminoacyl-tRNA ArgRS arginyl-tRNA synthetase BSA bovine serum albumin To whom correspondence should be sent |
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