Blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipoolysaccharide, lipid A and acid-degrade polysaccharide from Fusobacterium nucleatum |
| |
Authors: | Kjell Sveen Tor Hofstad |
| |
Institution: | Department of Microbiology and Immunology, Laboratory of Oral Microbiology, The Gade Institute, University of Bergen, Bergen, Norway |
| |
Abstract: | Abstract Lipopolysaccharide of Fusobacterium nucleatum strain Fevl was split by acid hydrolysis. The split products, i.e. lipid A and degraded polysaccharide were mitogenic for murine spleen cells as measured by uptake of 3H]thymidine. The uptake of 3H]thymidine was dose-dependent. Incubation of spleen cells with stimulants for 3 days resulted in a polyclonal activation of immunoglobulin synthesis. Higher mitogenic response and immunoglobulin production were found in spleen cells of athymic mice compared to those of thymic mice. The activity of lipid A in stimulating immunoglobulin synthesis was comparable with the parent lipopolysaccharide-Fevl, the degraded polysaccharide being the less potent stimulator. |
| |
Keywords: | Fusobacterium nucleatum Lipopolysaccharide Lipid A Polysaccharide Mitogenicity Immunoglobulin synthesis |
|
|