Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers |
| |
| Authors: | C Maliepaard F H Alston G van Arkel L M Brown E Chevreau F Dunemann K M Evans S Gardiner P Guilford A W van Heusden J Janse F Laurens J R Lynn A G Manganaris A P M den Nijs N Periam E Rikkerink P Roche C Ryder S Sansavini H Schmidt S Tartarini J J Verhaegh M Vrielink-van Ginkel G J King |
| |
| Institution: | (1) DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, 6700 AA, Wageningen, The Netherlands Fax: 31 317 41 80 94 E-mail: c.a.maliepaard@cpro.dlo.nl, NL;(2) Horticulture Research International, Wellesbourne (HRI-W), Warwick CV35 9EF, UK, GB;(3) Horticulture Research International, East-Malling (HRI-EM), West Malling, Kent ME19 6BJ, UK, GB;(4) Dipartimento di Colture Arboree (DCA), University of Bologna, Via Filippo Re 6, 40126, Bologna, Italy, IT;(5) Federal Centre for Breeding Research on Cultivated Plants, Institute for Ornamental Plant Breeding (IZZ), Bornkampsweg 31, D-22926, Ahrensburg, Germany, DE;(6) INRA, Station d’Amélioration des espèces fruitières et ornementales, Centre de Recherches d’Angers, 49070 Beaucouzé, France, FR;(7) NAGREF, Pomology Institute, PO Box 122, 592 00, Naoussa, Makedonia, Greece, GR;(8) Horticulture and Food Research Institute of New Zealand, Palmerston North Research Centre, Private Bag 11 030, Palmerston North, NZ, NZ;(9) Horticulture and Food Research Institute of New Zealand, Mt. Albert Research Centre, Private Bag 92 169, Auckland, NZ, NZ |
| |
| Abstract: | Linkage maps for the apple cultivars ‘Prima’ and ‘Fiesta’ were constructed using RFLP, RAPD, isozyme, AFLP, SCAR and microsatellite
markers in a ‘Prima’בFiesta’ progeny of 152 individuals. Seventeen linkage groups, putatively corresponding to the seventeen
haploid apple chromosomes, were obtained for each parent. These maps were aligned using 67 multi-allelic markers that were
heterozygous in both parents. A large number of duplicate RFLP loci was observed and, in several instances, linked RFLP markers
in one linkage group showed corresponding linkage in another linkage group. Distorted segregation was observed mainly in two
regions of the genome, especially in the male parent alleles. Map positions were provided for resistance genes to scab and
rosy leaf curling aphid (Vf and Sd
1, respectively) for the fruit acidity gene Ma and for the self-incompatibility locus S. The high marker density and large number of mapped codominant RFLPs and some microsatellite markers make this map an ideal
reference map for use in other progenies also and a valuable tool for the mapping of quantitative trait loci.
Received: 17 November 1997 / Accepted: 9 December 1997 |
| |
| Keywords: | Malus pumila Mill Molecular linkagemap Marker-assisted selection Fruit tree breeding Outbred progeny |
| 本文献已被 SpringerLink 等数据库收录！ |
|
