Identification of QTL underlying vitamin E contents in soybean seed among multiple environments |
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Authors: | Haiyan Li Huancheng Liu Yingpeng Han Xiaoxia Wu Weili Teng Guifeng Liu Wenbin Li |
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Institution: | (1) Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, Harbin, 150040, China;(2) Soybean Research Institute, Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030, China; |
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Abstract: | Vitamin E (VE) in soybean seed has value for foods, medicines, cosmetics, and animal husbandry. Selection for higher VE contents
in seeds along with agronomic traits was an important goal for many soybean breeders. In order to map the loci controlling
the VE content, F5-derived F6 recombinant inbred lines (RILs) were advanced through single-seed-descent (SSD) to generate a population including 144 RILs.
The population was derived from a cross between ‘OAC Bayfield’, a soybean cultivar with high VE content, and ‘Hefeng 25’,
a soybean cultivar with low VE content. A total of 107 polymorphic simple sequence repeat markers were used to construct a
genetic linkage map. Seed VE contents were analyzed by high performance liquid chromatography for multiple years and locations
(Harbin in 2007 and 2008, Hulan in 2008 and Suihua in 2008). Four QTL associated with α-Toc (on four linkage groups, LGs),
eight QTL associated with γ-Toc (on eight LGs), four QTL associated with δ-Toc (on four LGs) and five QTL associated with
total VE (on four LGs) were identified. A major QTL was detected by marker Satt376 on linkage group C2 and associated with
α-Toc (0.0012 > P > 0.0001, 5.0% < R
2 < 17.0%, 25.1 < α-Toc < 30.1 μg g−1), total VE (P < 0.0001, 7.0% < R
2 < 10.0%, 118.2 < total VE < 478.3 μg g−1). A second QTL detected by marker Satt286 on LG C2 was associated with γ-Toc (0.0003 > P > 0.0001, 6.0% < R
2 < 13.0%, 141.5 < γ-Toc < 342.4 μg g−1) and total VE (P < 0.0001, 2.0% < R
2 < 9.0%, 353.9 < total VE < 404.0 μg g−1). Another major QTL was detected by marker Satt266 on LG D1b that was associated with α-Toc (0.0002 > P > 0.0001, 4.0% < R
2 < 6.0%, 27.7 < α-Toc < 43.7 μg g−1) and γ-Toc (0.0032 > P > 0.0001, 3.0% < R
2 < 10.0%, 69.7 < γ-Toc < 345.7 μg g−1). Since beneficial alleles were all from ‘OAC Bayfield’, it was concluded that these three QTL would have great potential
value for marker assisted selection for high VE content. |
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