Identification and characterization of the gyrB gene from Treponema pallidum subsp. pallidum |
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| Authors: | Lola V Stamma Shermalyn R Greenea Natalie Y Barnesa |
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| Institution: | Department of Microbiology and Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa City, IA 52242, USA;G.W. Long Hansen's Disease Center at Louisiana State University, P.O. Box 25072, Baton Rouge, LA 70894, USA |
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| Abstract: | The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The organization of genes flanking the gyrB gene is unique in comparison to that of other bacteria. The gyrB gene encodes a 637 amino acid protein whose deduced sequence has a high degree of homology with type-II topoisomerase ATPase subunits (GyrB and ParE). Five type-II topoisomerase motifs, an ATP-binding site (Walker A), and amino acid residues that putatively interact with ATP, are highly conserved in the T. pallidum GyrB protein. |
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| Keywords: | Mycobacterium Nocardia F420 Glucose-6-phosphate dehydrogenase Streptomyces Corynebacterium Methanogenic Archaea |
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