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Synthesis of pentylferulate by a feruloyl esterase from Aspergillus niger using water-in-oil microemulsions |
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| Authors: | S. Giuliani C. Piana L. Setti A. Hochkoeppler P.G. Pifferi G. Williamson C.B. Faulds |
| Affiliation: | (1) Department of Industrial Chemistry, Viale del Risorgimento 4, 40136 Bologna, Italy;(2) Nutrition, Health and Consumer Sciences Division, Institute of Food Research, Norwich Research Park, Norwich, NR4 7UA, UK |
| Abstract: | Pentylferulate synthesis was achieved at high yields (50–60%) with Aspergillus niger feruloyl esterase using a water-in-oil microemulsion system. The initial rate of synthesis decreased by 15–20% when the water content of the microemulsion was increased from 1.8 to 2.4% (v/v), although a concomitant decrease in conversion was not observed. The enzyme stability was significantly higher in the microemulsion than in an aqueous solution. |
| Keywords: | Aspergillus niger enzyme esterification ferulic acid feruloyl esterase microemulsions |
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