A comparison of the effects of various stimulatory agents on t-PA secretion by normal and malignant cell lines |
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Authors: | J Clarke B Griffiths J Melling R Ling T Atkinson A Electricwala |
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Institution: | Division of Biologics, PHLS-Centre for Applied Microbiology and Research, Salisbury, Wilts, UK. |
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Abstract: | Succinyl con A and acetyl con A both stimulated epithelial cells to produce similar yields of tissue plasminogen activator (t-PA) to those previously obtained with native con A. However, unlike con A, the derivatized lectins did not adversely affect cell morphology and viability, and cells treated with succinyl con A could secrete t-PA for a prolonged period. Con A and the two derivatives produced similar morphological effects in Bowes melanoma cells, but t-PA production was not increased. Elevated cyclic nucleotide concentrations did not affect t-PA production from epithelial cells, but calcium ionophore treatment generated t-PA yields similar to those obtained with lectins. Azacytidine, which enhanced t-PA production from epithelial cells, did not increase yields from Bowes melanoma cells, and also sodium butyrate, reported to increase t-PA yields from human endothelial cells, had no effect on either cell line. |
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Keywords: | Tissue plasminogen activator epithelial cells Bowes melanoma cells concanavalin A succinyl concanavalin A acetyl concanavalin A cyclic nucleotides calcium ionophore butyrate azacytidine |
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