| Abstract: | Three anti-keratin MAbs were used to identify keratins expressed in early embryos of Bufo bufo gargarizans. MAb AF5 recognized three polypeptides of keratin in oooytes, fertilized eggs, up to neurula with Mr of 68, 65 and 60Kd respectively. At tailbud stage, three other keratins (62, 58 and 54Kd) began to express and could be detected by AP5. MAbs DIG and K12 gave different results, both of them could identify four keratin-like molecules with unusual molecular weights (Mr 98, 95, 30 and 27 Kd). Moreover, DIG could also detect a 54 Kd keratin in neurula and tailbud stage embryos, while K12 could reveal, beside 54Kd keratin, other four more keratins (68, 65, 62 and 60 Kd). The possible interpretation of these results and their implications are discussed. |
