| 新细胞系──粘虫胚胎细胞系的建立(英文) |
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| 引用本文： | 李国勋,于洪春,宋捷,李长友,王晓云. 新细胞系──粘虫胚胎细胞系的建立(英文)[J]. Entomologia Sinica, 1998, 0(1) |
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| 作者姓名： | 李国勋 于洪春 宋捷 李长友 王晓云 |
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| 作者单位： | 河北农业大学!保定，071001(李国勋)，东北农业大学!哈尔滨，150030(于洪春，宋捷，李长友，王晓云) |
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| 摘 要： | 一株新细胞系-粘虫（Mythimnaseparata）胚胎细胞系（NEAU－Ms－927311简称Ms937311）被建立。该细胞系多为梭形细胞，少数圆形，贴壁性强。细胞群体倍增时间为58．8h；接种3天后细胞增长速度加快，第5天达到最大值；染色体分析结果表明：染色体聚集成簇，棒球状，呈典型的鳞翅目昆虫细胞核型；酯酶同工酶分析有两条明显的谱带；该细胞系可被同源核型多角体病毒（MsNPV）侵染并形成多角体。
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NEW CELL LINE FROM EMBRYOS OF MYTHIMNA SEPARATA (LEPIDOPTERA:NOCTUIDAE) |
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| Guoxun Li. NEW CELL LINE FROM EMBRYOS OF MYTHIMNA SEPARATA (LEPIDOPTERA:NOCTUIDAE)[J]. Entomologia Sinica, 1998, 0(1) |
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| Authors: | Guoxun Li |
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| Abstract: | A new cell line was established from 2-d-old embryonated eggs of Mythimna separata and has been designated as NEAU-Ms-M927311. This cell line consists of a mixture of cell types , including spherical and spindle-shaped cells. The cell line has a population doubling tirne of 58. 8h.Chromosome analysis revealed its typical lepidopteran karyology. lsozyme characterization of esterase showed that the band pattern was different from that of other two cell lines (Xc-920730, and SF21AE). Virus infectivity tests revealed this cell line can support replication of M. separata nuclearpolyhedrosis virus. |
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| Keywords: | Mythimna separata embryonated eggs cell lines |
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